Transgenic Mouse Core
Director: Katia Georgopoulos
Location: Building 149 13th Street, Room 3111, Charlestown
| Core Summary: |
The Transgenic Mouse Core provides services which includes
microinjection of oocytes and
transplantation of embryos into foster recipients for the generation of transgenic
mice. ES cell injections and blastocyst transplantation to generate knockout
mice is also covered. Assistance is given to all investigators for their transgenic
related project functions, which include construct design, DNA preparation,
mouse handling, genotyping, and colony generation. The facility is also equipped
for the cryopreservation of 8-cell mouse embryos. Help is also provided to
other transgenic facilities within and outside MGH with troubleshooting in
problem areas.
| Personnel/Contact Information: |
Director: Katia Georgopoulos, Ph.D.
Phone: (617) 726-4445
Fax: (617) 726-4453
Email: katia.georgopoulos@cbrc2.mgh.harvard.edu
Other Staff:
Associate Director: Lin Wu, M.D., Ph.D.
Phone: (617) 724-8124
Fax: (617) 726-4453
Email: lin.wu@cbrc2.mgh.harvard.edu
| Facilities and Equipment: |
Location of Core:
The facility is located at Building 149 13th Street, Room 3111, Charlestown.
Major Equipment:
The facility is equipped for the cryopreservation of 8-cell mouse embryos.
| Services: |
►Construct
design
►DNA
preparation
►Mouse
handling
►Genotyping
►Colony
generation
►Microinjection
of oocytes and transplantation of embryos into foster recipients
for the generation of transgenic mice
►ES
cell injections and blastocyst transplantation to generate knockout
mice
| Fee Schedule/Rates: |
$2000 per project
If repeat is necessary, cost will be negotiated based upon time and materials
necessary.
| Getting Started: |
1. Contact Lin Wu at 724-8124 to
drop off sample (see below) and complete neccesary billing information.
2. Your lab will provide a DNA fragment to be injected to be free of the vector.
It is important to have a clean, pure and non-toxic DNA prep, and an accurate
DNA concentration in the final form prior to injection. You will also need
a concentration of approximately 50 ng/ul and a total volume of approximately
50 ul for the injection process.
3. Label your tube with:
a. the name of the construct
b. concentration of the fragment
c. size of the fragment
d. date
4. The transgenic facility will contact you as to the scheduling of the injections
and when the mice are born.
5. Prior to the mice being born, you will be contacted by the transgenic core
to complete the necessary paperwork to have the mice transferred to the general
facility.
| Links to other outside Academic and Commercially Available Services: |
| Institution | Contact | Website |
| BWH | Arlene Sharpe (617) 278-0312 |
http://research.bwh.harvard.edu |
| Tufts Univeristy | Janis Lem jlem@tufts-nemc.org |
http://www.nemc.org |
| BIDMC | Joel Lawitts (617) 632-0264 |
http://research.bidmc.harvard.edu/CoreLabs/trans.asp |
| MIT | Aurora Connor (617) 452-3658 |
http://web.mit.edu/transgenic/ |
| Johns Hopkins | Chip Hawkins (410) 614-3858 |
http://www.hopkinsmedicine.org/core/Home.htm |
| Departmental Web Link: |
