Nucleic Acid Quantitation Core (Real-Time PCR)
Director: Samuel Rabkin
Location: Building 149 13th Street, Room 6029, Charlestown
| Core Summary: |
The Nucleic Acide Quantification Core (Real-Time PCR) offers quantitative measurement of DNA or RNA levels using real-time PCR.
| Personnel/Contact Information: |
Director: Samuel Rabkin, Ph.D.
Phone: (617) 726-6817
Fax: (617) 643-3422
Email: rabkin@helix.mgh.harvard.edu
Other Staff:
Research Technician: Luba Zagachin
Phone: (617) 726-9587
Fax: (617) 726-7864
Email: lzagachin@partners.org
| Facilities and Equipment: |
Location of Core:
The facility is located at Building 149 13th Street, Room 6029, Charlestown.
Major Equipment:
►Real-time
quantitative PCR machine (ABI Prism 7000 Sequence Detector System)
►Software
for primer selection and data analysis
►Automated
nucleic acid purification and processing (ABI Prism 6100 Nucleic Acid PrepStation)
| Services: |
Real-time quantitative PCR provides improved sensitivity,
accuracy, speed, and throughput
over standard PCR methods. The ABI Prism 7000 is a new instrument that replaces
the
ABI 7700, which has been a standard for real-time PCR. It has a detection range
of over 5 logs in a 96 well format. Detection is based on TaqMan fluorescent
probes with 5' reporter and 3' quencher labels, such that synthesis from flanking
primers leads to cleavage of the 5' reporter dye and detectable fluorescence.
TaqMan probes can be synthesized with different color dyes permitting the analysis
of multiple sequences in a single PCR reaction, so that each reaction can contain
an internal control. The TaqMan probes can also be used for allelic discrimination
assays. SYBR green can be used in place of the TaqMan probes to detect total
PCR product.
| Fee Schedule/Rates: |
| Service | MGH |
| Laser Capture Microscopy | $50/hour |
| Getting Started: |
To access the core, please contact Luba
Zagachin at (617) 726-9587 or by email at
lzagachin@partners.org.
| Departmental Web Link: |
http://www.massgeneral.org/ncs/neuro_core_NucleicAcidQuantitation.htm
