The Monoclonal Antibody Production Facility provides a monoclonal antibody production service to research projects. Protein/peptides provided by the individual investigators will be used to generate mouse monoclonal antibodies.

Director: Vijaya Ramesh, Ph.D.
Phone: (617) 724-9733
Fax: (617) 726-3655
Email: ramesh@helix.mgh.harvard.edu

Other Staff:

Senior Research Technician: Nicole Smith (Team Leader)
Phone: (617) 724-0841
Email: nsmith5@partners.org

Research Technician: Carolyn Polizzano
Phone: (617) 724-9559
Email: cpolizzano@partners.org

Location of Facility:

The facility is located at 185 Cambridge Street, Simches Building, CPZN-5-5398.

Major Equipment:

Tissue culture hoods and incubators
Centrifuge
Quixell cell transfer system for hybridoma clone selection
-80 degree freezer and liquid N2 freezers

• The facility will provide consultation to investigators in choosing the appropriate peptides or expressing the protein of interest as bacterial fusion proteins.
• The facility will be responsible for ordering mice, injecting peptides/proteins and testing the mouse sera for positive response.
• Hybridoma fusion will be carried out on one or more animals depending on the response,
  and screening will be performed by ELISA and Western blot analyses.
• A systematic isolation of positive clones will be performed using the Quixell Cell Transfer
  System, and the validity of hybridoma clones will once again be tested by ELISA and Western blots. In consultation with the investigators best clones will be expanded, and concentrated supernatants will be produced. If necessary we will arrange for Ascites production.

To access the resource, please contact Nicole Smith at (617) 724-0841 or by email at
nsmith5@partners.org.

http://www.massgeneral.org/ncs/neuro_core_Monoclonalantibodyproduction.htm