The Microscopy Core of the Program in Membrane Biology
(PMB) at MGH is equipped and staffed to provide a wide range of services
to investigators from MGH and the Boston area scientific community in
the area of light and electron microscopy. The Core is housed on the
8th floor of the Simches Research Center at MGH, and is directed by Dr.
Dennis Brown, Ph.D. The Core will provide services ranging from complete
performance of the technical procedures required, to full training of
personnel. Among the techniques available are laser scanning confocal
microscopy, spinning disk confocal microscopy, tissue fixation, sectioning,
immunostaining and conventional immunofluorescence microscopy, image
analysis, and all aspects of electron microscopy including immunogold
staining. The Core facility provides a unique and important resource
for members of the scientific community as they approach a variety of
research problems that call for these advanced microscopy techniques.
Director: Dennis Brown, Ph. D.
Phone: (617) 726-5665
Fax: (617) 643-3182
Senior Lab Technologist: Robert Tyszkowski (cryostat sectioning; confocal microscopy)
Fax: (617) 643-3182
Senior Lab Technologist: Mary McKee (ultrathin sectioning; electron microscopy)
Fax: (617) 643-3182
Location of Core:
The facility is located at the Program in Membrane Biology,
8th Floor, MGH Simches Research Center, 185 Cambridge Street, Boston.
- JEOL 1011 electron microscope with fully digital image capture and
- BioRad/Zeiss Radiance 2000 confocal microscope
- Perkin Elmer LCI Spinning Disk Confocal Microscope
- Nikon Total Internal Reflection Fluorescence (TIRF)
- Nikon E800 Eclipse upright epifluorescence microscope with Hamamatsu
Orca CCD camera and complete image analysis software (IP Lab)
- Nikon 80i upright epifluorescence microscope with Hamamatsu Orca
CCD camera and complete image analysis software (IP Lab)
- Nikon TE300 inverted microscope with wide-field optics and Hamamatsu
Orca CCD camera for fluorescence and ratio imaging, with Improvision
- Olympus BH2 upright microscope with Spot Insight color CCD camera
and Macintosh G4 computer for light microscopy
- Olympus doubleheaded light microscope for section examination
- Reichert ultramicrotomes for routine EM sections (2)
- Leica FCS ultracryomicrotome for ultrathin frozen sectioning
- Leica cryostats (2)
- Leica microtome for paraffin sectioning
- MMI CellCut laser microdissection system
- Pelco 101 Vibratome
- Leica EM AFS2 freeze substitution system
- Several Macintosh G4-based computer workstations equipped with IP
Lab Spectrum software for image analysis
- 2 Power Mac G5 based workstations with Improvision Velocity visualization,
classification (morphometric analysis) and restoration (digital deconvolution)
software, 8 gigabytes of RAM, and 256 mb Radeon video cards
1. Technical Services
The core will perform conventional immunofluorescence microscopy, confocal
microscopy, live-cell imaging, conventional electron microscopy and EM
immunogold labeling, tissue sectioning, tissue fixation, and computer
based image analysis and quantification. In some cases, investigators
will provide their own stained tissues/cell for imaging, while in others
the Core will perform all services from fixation and sectioning, to staining
2. Training Services
The core technicians will also provide training to high frequency users
who wish to invest the substantial time necessary for learning to use the
equipment proficiently (with the exception of the electron microscope).
3. Consultation Services
Dr. Dennis Brown and the Core technologists will be available for consultation
in the use of the different microscopy techniques, and to provide technical
advice, such as the different fixation techniques that enable antibodies to
detect their antigens.
4. Development for Core Enhancement
Dr. Dennis Brown and the advisory committee will continually explore
new techniques to be offered as part of the core service.
Please inquire with the Core.
To access the core, please contact the appropriate persons above (Dennis
Brown for general inquiries, Robert Tyszkowski for light and confocal
microscopy, and Mary McKee for EM work and sectioning).