The Cell Culture and Microscopy Core provides well-characterized
primary and experimentally immortalized human cell lines, custom culture
services, and training and access to a conventional fluorescence microscope
and digital camera.
| Personnel/Contact
Information: |
Director: James Rheinwald, Ph.D.
Phone: (617) 525-5553
Fax: (617) 525-5571
Email: jrheinwald@rics.bwh.harvard.edu
Other Staff:
Core Manager: Jay Omobono
Phone: (617) 525-5562
Fax: (617) 525-5571
Email: jomobono@partners.org
| Facilities
and Equipment: |
Location of Core:
The facility is located at 77 Avenue Louis Pasteur,
HIM 660, Boston.
Major Equipment:
Cell culture laboratory; Nikon phase contrast and Nomarski
optics microscope equipped
for fluorescence microscopy and digital photography.
Cell Culture Services
►Well-characterized
normal primary human epidermal, oral, and ectocervical keratinocyte,
connective tissue fibroblast, large vessel endothelial, and pleural mesothelial
cell lines and
cell lines cultured from squamous cell carcinomas.
►Initiation
of primary fibroblast and keratinocyte lines from biopsies of human tissue
(e.g.,
generation of primary cell lines from individuals known to carry a specific
gene mutation).
►Genetic
engineering of such cells using retroviral or lentiviral vectors.
►Cryopreservation
and storage of cultures.
►Provision of
optimized culture medium formulations for various cells types.
►Training
in cell culture and experimental design using human cell lines.
►Consultation
and custom culture services.
Microscope Services
►Instruction
in the use of and access to our phase contrast/fluorescence microscope,
digital
camera, and computer with photo-editing software.
Cell Culture Fee Schedule
| Service |
Unit
Price |
Normal, primary human cell lines (keratinocytes,
fibroblasts, mesothelial cells, etc.), squamous cell carcinoma (SCC)
cell lines
and HPV16E6E7- or TERT-immortalized cell lines. |
$150/ampule
$200/ampule (overseas shipment) |
| T25 Flask culture of cells with 50ml medium,
10ml trypsin/EDTA, 10ml neutralization medium, and 5ml 2X freezing
medium (for pickup at Core lab; no shipments). |
$250 |
| Specialized culture medium. |
$35/100ml bottle |
| Pre-tested, selected lots of calf serum. |
$25/100ml botte |
| Initiation of new keratinocyte or fibroblast cell
lines from biopsy. |
$1000, plus ampule/flask charges |
| Preparation of TERT- or HPV16/E6E7-immortalized
lines or other genetically engineered human cell lines, by retroviral
or transduction. |
$1000, plus ampule/flask charges |
Microscope/Camera Fee Structure
| Training and registration |
$100 |
| Use of equipment |
$30/hour |
To access the core, please visit http://rheinwaldlab.bwh.harvard.edu/core.html.
| Links
to other outside Academic and Commercially Available Services: |
http://rheinwaldlab.bwh.harvard.edu/core.html
|
