Gel Scanning Facility Core
Director: José Romero
Location: 221 Longwood Avenue, EBRC-215, Boston

 
 
Core Summary:

The Gel Scanning Core is a high performance gel and blot imager with the capability of microarray analysis. It offers three methods of detection: storage phosphor, fluorescence and chemiluminescence. This imager unites proven storage phosphor autoradiography technology with four-color, non-radioactive fluorescent labeling techniques. For DNA, RNA, and protein samples, choose from:

  • Storage phosphor autoradiography
  • Direct blue-excited fluorescence (457, 488 nm)
  • Direct green-excited fluorescence (532 nm)
  • Direct red-excited fluorescence (633 nm)
  • Chemiluminescence

When one of the scanning modes is selected, the appropriate optical components are
automatically activated. Typhoon™ scans gels, blots, and mounted or unmounted storage
phosphor screens up to 35 × 43 cm. The instrument exhibits outstanding linearity,
quantitative accuracy, and extremely low limits of detection.

The Typhoon™ 9410 Variable Mode Imager and PC workstations are available to any Principal Investigator in the Longwood Medical Area. Typhoon 9410 Workstation

Personnel/Contact Information:

Director: José Romero, Ph.D.
Phone: (617) 732-4948
Fax: (617) 732-5764
Email: jrromero@rics.bwh.harvard.edu

Other Staff:

Tham Yao
Fax: (617) 732-5764
Email: tyao@partners.org

Facilities and Equipment:

Location of Core:

The facility is located at 221 Longwood Avenue, EBRC-215, Boston.

Major Equipment:

Typhoon 9410 Workstation from GE Healthcare (Formerly Amersham Biosciences) - Universal gel, blot and microarray imager with blue excitation source with two PC workstations.

Services:

The Typhoon 9410 workstation is available to all Principal Investigators within the Longwood Medical Research Area. The scanning facility is self-serviced. We provide basic initial instruction on its use. The PC workstation has a CD writer and a ZIP250 MB disk drive for your use. The Typhoon is not networked to the Hospital or internet. The Typhoon 9410 imager:

Versatile system handles gel sandwiches, agarose and polyacrylamide gels, membranes,
microplates.
Powerful excitation sources and innovative high-quality confocal optics allow for the
sensitive detection of low-abundance targets.
Red-, green-, and blue-excitation wavelengths and a wide choice of emission filters enable
imaging of an extensive variety of fluorophores.
Automated four-color fluorescence scanning allows multiplexing of multiple targets in the
same sample ensuring accuracy of analysis, increasing throughput, and saving time.
Storage phosphor technology delivers high-resolution imaging and accurate quantitation
of 3H, 14C, 125I, 32P, 33P, 35S, and other sources of ionizing radiation.
Highly sensitive optics enables direct chemiluminescent imaging without intermediate
exposure to films or screens.

Fee Schedule/Rates:

Service
BWH
Non-BWH
Industry
Scanning Cost
$2.25/min
$2.75/min
$4.00/min

Getting Started:

Any Principal Investigator within the Longwood Medical Area is welcome to use this Core
Facility. To access the core, please contact Dr. Jose R. Romero by email at:

jrromero@rics.bwh.harvard.edu

 

Upon receipt of your email request, you will be sent an application form. After receipt of this application form you will be provided with a personal Login and Password to access the Typhoon PC workstation in EBRC-215.


 
 
     
 

 

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