The Histopathology and Image Analysis Core facility
provides a wide array of histology services, including sample processing,
embedding and sectioning; routine chemical stains such as H&E, trichrome,
and PAS; specialized chemical stains such as Sirius Red; immunostaining,
and image analysis/morphometric analyses.
| Personnel/Contact
Information: |
Director: Kathleen Haley, M.D.
Phone: (617) 732-4942
Fax: (617) 232-4623
Email: khaley@partners.org
Other Staff:
Research Assistant: Susanna Minasyan
Phone: (617) 732-7112
Fax: (617) 232-4623
Email: sminasyan@partners.org
| Facilities
and Equipment: |
Location of the Core:
The facility is located at 75 Francis Street, Thorn
Building, Room 910, Boston.
Major Equipment:
ThermoShandon tissue processing and embedding equipment
Leica microtome
Leica light and fluorescent microscope
| Service |
BWH |
| Tissue Cassetting (grossing) routine |
$3 |
| Tissue Cassetting (grossing) small size |
$4 |
| Processing |
$3 |
| Processing for culture plate samples |
$50/6–well
plate |
| Embedding - unspecified orientation |
$3 |
| Embedding - sagittal cuts and/or orientation |
$5 |
| Embedding - embryonic sample or < 5 mm |
$5 |
| Block facing |
$2.50 |
| 5-micron paraffin section |
$2 |
| Sequential paraffin sectioning |
$2 |
| Step sectioning |
$3 / “step”,
$2 / section |
| Serial paraffin sections (not sequential) |
$2 |
| Chemical Stains (price per slide) |
| H&E |
$2 |
| Gill's hematoxylin |
$2 |
| Trichrome |
$10 |
| PAS |
$10 |
| Elastin |
$15 |
| Esterase |
$7.50 |
| Apoptag (price per slide) |
| Apoptag (core kit) |
$45 |
| Apoptag (PI kit) |
$30 |
| Immunostaining (price per slide) |
| Immunostain - known/optimized AB, core provide |
$35 |
| Immunostain - known AB, PI provide paraffin |
$25 |
| Immunostain - known AB, PI provided frozen |
$25 |
| Immunostain - optimization of know AB for fix |
$35 |
| Immunostain - optimization of known AB for tissue |
$35 |
| Immunostain - new AB, PI provided |
$35 |
| Immunostain – new AB, core provided |
$40 |
| Miscellaneous Services |
| Photomicrography by core personnel |
$100/hour |
| Morphometric analysis by core personnel |
$100/hour |
| Training |
$50/hour |
| Consultation with histology services |
$50/hour |
| Consultation without histology services |
$50/hour |
| Consultation regarding morphometric analyses |
$50/hour |
| Unassisted Equipment Use |
| Processor |
$50/run |
| Embedding machine |
$5/hour |
| Microtome |
$20/hour |
| Image analysis |
$20/hour |
| Chemical stains |
$45/run |
| Immunostains |
$75/run |
To access the core, please contact Kathleen Haley at
(617) 732-4942 or by email at
khaley@partners.org.
|
