MGH Pathology Simches and CNY Flow Cytometry Core and Flow Image Analysis

Director: Frederic Preffer, Ph.D.
Location: Massachusetts General Hospital
Boston Campus Location: Simches 3.434
Charlestown Navy Yard Location: CNY 5.226


 
 
Core Summary:

The cell sorting and analysis lab provides access to the most sophisticated flow cytometers presently available. The core is able to analyze and physically separate cells based upon features detectable with fluorescently labeled monoclonal antibodies. The Amnis technology combines flow with fluorescent and bright field image-based analysis.

Personnel/Contact Information:

Director: Frederic Preffer, Ph.D.
Phone: (617) 726-7481
Fax: (617) 724-3164
Email: preffer@helix.mgh.harvard.edu

Other Staff:

Senior Research Technician, Simches site:
David Dombkowski
Phone: (617) 726-1683
E-mail: dombkowski@helix.mgh.harvard.edu

Core Lab Technology Scientist, Amnis Imaging Flow Cytometry, Simches Site:
Scott Mordecai
Phone: (617) 726-1683

E-mail: smordecai@mgh.harvard.edu

Senior Research Technician, CNY site:
Ravi Mylvaganam, Ph.D.
Phone: (617) 643
-6771
E-mail: rmylvaganam@mgh.harvard.edu

Research Technician, CNY site:
Christina Luo
Phone: (617) 643
-6771
E-mail: ccluo@mgh.harvard.edu

Facilities and Equipment:

The Simches site is located in room 3.434 in the Simches Building, MGH Boston.

Major Equipment (Simches):

  • Modified BD Digital Vantage SE Cell Sorter with one water cooled Argon Laser (488 nm, 514 nm, 457 nm), three water cooled Krypton Lasers (355 nm, 568 nm, 407 nm) and one air cooled 633 nm laser.
  • SORP 8 Laser (355 nm, 405 nm, 445 nm, 488 nm, 532 nm, 640 nm, 785 nm, 592 nm) BD FACSAria III Fusion cell sorter, BSL2+.
  • Analytic SORP 8 Laser (355 nm, 405 nm, 488 nm, 532 nm, 552 nm, 594 nm, 640 nm, 785 nm) BD LSR II.
  • Amnis ImageStreamx mkII Imaging Flow Cytometer configured with 405 nm, 488 nm, 561 nm, 592 nm, and 642 nm lasers. This dual camera 12-channel instrument has 20x, 40x and 60x Magnification, EDF and 96-well plate capability.

The Charlestown Navy Yard (CNY) site is located in room 5.226 in CNY Building 149, 13th Street, Charlestown Navy Yard.

Major Equipment (CNY):

  • Analytic SORP 4 Laser (405 nm, 488 nm, 561 nm, 640 nm) BD LSR II
  • Analytic SORP 4 Laser (405 nm, 488 nm, 561 nm, 640 nm) BD Fortessa
  • SORP 5 Laser (355 nm, 405 nm, 488 nm, 56 1nm, 640 nm) BD FACSAria II cell sorter
  • SORP 5 Laser (355 nm, 405 nm, 488 nm, 561 nm, 640 nm) BD FACSAria Fusion cell sorter, BSL2+
Services:

State of the art multiparametric flow cytometric analysis, cell sorting, and flow-based imaging analysis.

FY15 User Fees (Effective 10/1/14):

Service
Internal Users
External Users
Partners
Non Partners AMC & Non-profit
For-Profit & Industry
Digital FACSVantage - Simches
$76
$110
$250
LSRII - Simches
$72
$104
$250
FACSAria - CNY
$81
$117
$250
LSRII with operator - CNY
$76
$110
$250
LSRII without operator - CNY
$47
$68
$250
Fortessa w/out operaor-CNY
$47
$68
$250
Fortessa with operator - CNY
$76
$110
$250
Amnis - Simches
$70
$101
$250
Technician Time
$70
$101
$250

Getting Started:

To access the core, please contact Dr. Frederic Preffer at (617) 726-7481 or by email at preffer@helix.mgh.harvard.edu. Alternatively, you can contact David Dombkowski at (617) 726-1683 or by email at dombkowski@helix.mgh.harvard.edu for the Simches facility or Ravi Mylvaganam, Ph.D. at (617) 643-6771 or by email at rmylvaganam@mgh.harvard.edu for the CNY facility.

For questions about the Amnis technology please contact Scott Mordecai at (617) 726 -1683 or by email at smordecai@mgh.harvard.edu.

 
 
     
 

 

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