Flow Cytometry Core
Director: Frederic Preffer
Locations: 185 Cambridge Street, Room 3.434, Simches Building, Boston
Building 149 13th Street, Room 6307, Charlestown
| Core Summary: |
The cell sorting and analysis lab provides access to the most sophisticated flow cytometers presently available. The core is able to analyze and physically separate cells based upon features detectable with fluorescently labeled monoclonal antibodies.
| Personnel/Contact Information: |
Director: Frederic Preffer, Ph.D.
Phone: (617) 726-7481
Fax: (617) 724-3164
Email: preffer@helix.mgh.harvard.edu
Other Staff:
Senior Research Technician, Simches site: David Dombkowski
Phone: (617) 726-1683
Email: dombkowski@helix.mgh.harvard.edu
Senior Research Technician, CNY site: Ravi Mylvaganam, Ph.D.
Phone: (617) 643-6771
Email: rmylvaganam@partners.org
| Facilities and Equipment: |
The Simches site is located in the newly constructed space located in room 3.434 in the Simches Building.
Major Equipment (Simches):
- Analytic Seven Laser LSR configured with 355 nm, 407 nm, 488 nm, 532 nm, 594 nm, 633 nm, and 780 nm air cooled lasers providing a wide range of excitation possibilities.
- Modified Digital Vantage cell sorter with 2 water-cooled argon, 2 krypton and one air cooled 633 nm laser.
The CNY site is located in the newly constructed space located in room 6.307 in Building 149, 13th Street, Charlestown Navy Yard.
Major Equipment (CNY):
- Analytic three Laser LSR II configured with 405 nm, 488 nm, and 633 nm air cooled lasers providing a wide range of excitation possibilities.
- A four laser FACSAriaII cell sorter configured with 405 nm, 488 nm, 594 nm and 633 nm air cooled lasers providing a wide range of excitation and sorting possibilities.
| Services: |
State-of-the-art multi-parametric flow cytometric analysis and cell sorting.
| FY12 User Fees (Effective 10/1/11): |
| Service | Internal Users |
External Users |
|
Partners |
Non Partners AMC & Non-profit
|
For-Profit & Industry
|
|
| &Digital FACSVantage - Simches | $70 |
$100.80 |
$250
|
| FACSCalibur - Warren | $25 |
$43 |
- |
| LSRII - Simches | $65 |
$93.60 |
$250 |
| FACSAria - CNY | $70 |
$100.80 |
$250 |
| LSRII with operator - CNY | $65 |
$93.60 |
$250 |
| LSRII without operator - CNY | $40 |
$57.60 |
$250 |
| Getting Started: |
To access the core, please contact Dr. Frederic Preffer at (617) 726-7481 or by email at preffer@helix.mgh.harvard.edu. Alternatively, you can contact David Dombkowski at
(617) 726-1683 or by email at dombkowski@helix.mgh.harvard.edu for the Simches facility or Ravi Mylvaganam, Ph.D. at (617) 643-6771 or by email at rmylvaganam@partners.org.
