Flow Cytometry Core

Director: Ronglih Liao, Ph.D
Location:
77 Avenue Louis Pasteur, NRB 435E, Boston


 
 
Core Summary:

To provide high standard cell sorting and analysis services to the research community at the Brigham and Women’s Hospital and the Longwood Medical area.

Personnel/Contact Information:

Director:
Director: Ronglih Liao, Ph.D.
Phone: (617) 525-4854
Fax: (617) 525-4868
Email: rliao@rics.bwh.harvard.edu

Other Staff:
Core Manager/Staff Scientist: Yiling Qiu, Ph.D.
Phone: (617) 525-4866, (617) 525-4011
Fax: (617) 525-4868
Email: yqiu@partners.org

Facilities and Equipment:

Location of Core: 
77 Avenue Louis Pasteur, NRB 435E, Boston

Major Equipment:  
BD FACSAria Multicolor High Speed Sorter
BD Accuri C6 4-color Analyzer
Cytek DxP12 10-color Analyzer

Services:
  • Sorting (up to 10 colors) of multicolor immunophenotyped samples.
  • Sorting and analysis of GFP, YFP, mTomato, gene expression proteins.
  • Calcium flux measurements using Indo-1.
  • DNA analysis using standard dyes (Hoeschst, DAPI, PI, 7-AAD, etc.).
  • Sorting stem cell side populations using Hoechst.
  • Single cell sorting (cloning) into 96-well plates.
  • Sorting up to 4 populations at once into Eppendorf, 5 ml or 15 ml tubes.
  • And more…
FY15 User Fees (Effective 10/01/14):

Services
Internal Users
External Users
Partners
Non Partners AMC & Non-Profit
For-Profit & Industry
Aria Sorter
$90.00
$130.00
Contact Core
Accuri C6 Analyzer
$35.00
$50.00
Contact Core
BD DXP 10 Sorter
$47.00
$68.00
Contact Core

 

Cancellation Policy: In order to maintain availability of heavily scheduled instruments, all reservations must be canceled at least 24 hours in advance on the previous business day to avoid being charged. A cancellation for the first working day of a week must be done on the last working day of the previous week. If the Core can find a productive use of the cancelled time (most likely filling it at the last moment), there will be no charge.

Getting Started:

For more information on the core, please visit the website at:
http://bwhbri.partners.org/BWH_Flow_Cytometry_Core/Home_page.asp

Online sign-up:
Please register first with Yiling Qiu at yqiu@partners.org.

Sample Preparation:

What samples should I bring?

  • All users must always bring single stained compensation samples each stained with one color AND one unstained tube. Cell suspensions should be passed through a 35um nylon mesh filter (BD Biosciences part # 352235) right before sorting.
  • Don’t have enough cells for compensation?
    User can purchase compensation beads from BD BD CompBeads 552845 ‘Anti-Rat/Hamster’ for mouse samples BD CompBeads 552843 ‘Anti-Mouse’ for almost all human samples.

Departmental Web Link :

http://bwhbri.partners.org/BWH_Flow_Cytometry_Core/Home_page.asp

 

 
 
     
 

 

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