To provide high standard cell sorting and analysis services to the research community at the Brigham and Women’s Hospital and the Longwood Medical area.
| Personnel/Contact
Information: |
Director: Ronglih Liao, Ph.D.
Phone: (617) 525-4854
Fax: (617) 525-4868
Email: rliao@rics.bwh.harvard.edu
Other Staff:
Core Manager/Operator: Grigoriy Losyev
Phone: (617) 525-4831
Fax: (617) 525-4868
Email: glosyev@partners.org
| Facilities
and Equipment: |
Location of Core:
77 Avenue Louis Pasteur, NRB 435E, Boston
Major Equipment:
BD FACS Aria multicolor high speed sorter
- Sorting (up to 10 colors) of multicolor immunophenotyped samples.
- Sorting and analysis of GFP, YFP, mTomato, gene expression proteins.
- Calcium flux measurements using Indo-1.
- DNA analysis using standard dyes (Hoeschst, DAPI, PI, 7-AAD, etc.).
- Sorting stem cell side populations using Hoechst.
- Single cell sorting (cloning) into 96-well plates.
- Sorting up to 4 populations at once into Eppendorf, 5 ml or 15 ml tubes.
- And more…
| Services |
BWH |
Non-BWH |
Sorting or Analysis w/operator
(billable in 30 minute increments) |
$65/hr |
$120/hr |
| Cancellation Policy: In order to maintain availability of heavily scheduled instruments, all reservations must be canceled at least 24 hours in advance on the previous business day to avoid being charged. A cancellation for the first working day of a week must be done on the last working day of the previous week. If the Core can find a productive use of the canceled time (most likely filling it at the last moment), there will be no charge. |
For more information on the core, please visit the website at:
http://bwhbri.partners.org/BWH_Flow_Cytometry_Core/Home_page.asp
Online sign-up:
Please register first with Grigoriy Losyev at glosyev@partners.org.
Sample Preparation:
What samples should I bring?
- All users must always bring single stained compensation samples each stained with one color AND one unstained tube. Cell suspensions should be passed through a 35um nylon mesh filter (BD Biosciences part # 352235) right before sorting.
- Don’t have enough cells for compensation?
User can purchase compensation beads from BD BD CompBeads 552845 ‘Anti-Rat/Hamster’ for mouse samples BD CompBeads 552843 ‘Anti-Mouse’ for almost all human samples.
| Links
to other outside Academic and Commercially Available Services: |
http://bwhbri.partners.org/BWH_Flow_Cytometry_Core/Home_page.asp
|
