Neuroscience Microscope Core
Director: Cristopher Bragg
Location: Building 149 13th Street, Room 6028, Charlestown
| Core Summary: |
The Neuroscience Microscope Core offers laser confocal microscopy services.
| Personnel/Contact Information: |
Director: Cristopher Bragg, Ph.D.
Phone: (617) 643-5754
Fax: (617) 726-6656
Email: dbragg1@partners.org
Other Staff:
Research Technologist: Igor Bagayev
Phone: (617) 724-0588
Fax: (617) 726-5677
Email: ibagayev@partners.org
| Facilities and Equipment: |
Location of Core:
The facility is located at Building 149, 13th Street, Room 6028B, Charlestown.
Major Equipment:
This Core consists of a Zeiss LSM 5 Pascal laser confocal microscope. The Zeiss RGB vario laser module consists of argon laser (458/488/514 nm) and two helium-neon lasers (543 and 633 nm). A Zeiss Axiovert 200 fully motorized light microscope is available with fluorescence, bright-field, phase-contrast and Nomarski (DIC) capabilities. Image acquisition and analyses are performed using Zeiss LSM 5 Pascal Confocal Microscopy Software (Release 3.2) on 2 workstations. Zeiss Physiology software is available also. Live cell imaging is available using a Zeiss temperature controller with custom chamber and heating stage.
| Services: |
► Double-
and triple-labeling experiments
►High-resolution
images (2048 x 2048 pixels)
►Quantitative
information for each channel
►3-Dimensional
reconstruction
►Localized
scans and FRAP (fluorescence recovery after photo-bleaching)
►Intracellular
ion concentration
►Intracellular
transport mechanism
►Dynamic
events in live cells and tissues
| FY13 User Fees (effective 10/1/12-9/30/13): |
Service |
Internal Fee: Neuroscience Users |
Internal Fee: Other Partners Users |
External Fee: Non-Partners AMC and Non-profit |
Minimum External Fee: For-profit and Industry* |
Ziess Confocal, Assisted, Per Hour |
$57.00 |
$90.00 |
$130.00 |
$144.00 |
Ziess Confocal, Unassisted, Per Hour |
$57.00 |
$61.00 |
$88.00 |
$97.00 |
Training, Per Hour |
$57.00 |
$90.00 |
$130.00 |
$144.00 |
| Getting Started: |
To access the core, please contact Igor Bagayev
at (617) 724-0588 or by email at
ibagayev@partners.org.
| Links to other outside Academic and Commercially Available Services: |
| Institution | Contact | Website |
| BIDMC | Daniel Brown (617) 667-4188 |
http://research.bidmc.harvard.edu/Corelabs/Microscopy.asp |
| University of Massachusetts | Chris Woodcock (413) 545-3751 |
http://www.bio.umass.edu/microscopy/index.html |
| Tufts University | Ron Willson (617) 636-6607 |
http://www.tufts.edu/sackler/cmdb/core_research.htm |
| Harvard University | Jamin DeProto (617) 432-3108 |
http://www.hms.harvard.edu/core/cb_confocal.html |
| Johns Hopkins University | Douglas Murphy (410) 955-4536 |
http://www.hopkinsmedicine.org/micfac/ |
| Departmental Web Link: |
http://www2.massgeneral.org/ncs/neuro_core_ConfocalMicroscope.htm
