Neuroscience Microscope Core

Directors: Cristopher Bragg
Locations: Building 149 13th Street, Room 6028, Charlestown


 
 
Core Summary:

The Neuroscience Microscope Core offers laser confocal microscopy services.

Personnel/Contact Information:

Director: Cristopher Bragg, Ph.D.
Phone: (617) 643-5754
Fax: (617) 726-6656
Email: dbragg1@partners.org

Other Staff:

Research Technologist: Igor Bagayev
Phone: (617) 724-0588
Fax: (617) 726-5677
Email: ibagayev@partners.org

Facilities and Equipment:

Location of Core:

The facility is located at Building 149, 13th Street, Room 6028B, Charlestown.

Major Equipment:

This Core consists of a Zeiss LSM 5 Pascal laser confocal microscope. The Zeiss RGB vario laser module consists of argon laser (458/488/514 nm) and two helium-neon lasers (543 and 633 nm). A Zeiss Axiovert 200 fully motorized light microscope is available with fluorescence, bright-field, phase-contrast and Nomarski (DIC) capabilities. Image acquisition and analyses are performed using Zeiss LSM 5 Pascal Confocal Microscopy Software (Release 3.2) on 2 workstations. Zeiss Physiology software is available also. Live cell imaging is available using a Zeiss temperature controller with custom chamber and heating stage.

Services:

Double- and triple-labeling experiments
High-resolution images (2048 x 2048 pixels)
Quantitative information for each channel
3-Dimensional reconstruction
Localized scans and FRAP (fluorescence recovery after photo-bleaching)
Intracellular ion concentration
Intracellular transport mechanism
Dynamic events in live cells and tissues

FY15 User Fees (effective 10/1/14)

Service
Internal Fee: Neuroscience Users
External Fee: Non-Partners AMC and Non-profit

 

Fee: For-profit and Industry*

Ziess Confocal, Assisted, Per Hour
$100
$144
$159
Ziess Confocal, Unassisted, Per Hour
$80
$115
$127
Training, Per Hour
$90
$130
$143

Getting Started:

To access the core, please contact Igor Bagayev at (617) 724-0588 or by email at
ibagayev@partners.org.

Links to other outside Academic and Commercially Available Services:

 
Institution
Contact
Website
BIDMC
Daniel Brown
(617) 667-4188
University of Massachusetts
Chris Woodcock
(413) 545-3751
Tufts University
Ron Willson
(617) 636-6607
Harvard University
Jamin DeProto
(617) 432-3108
Johns Hopkins University
Douglas Murphy
(410) 955-4536

Departmental Web Link:

http://www2.massgeneral.org/ncs/neuro_core_ConfocalMicroscope.htm

 

 
 
     
 

 

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