MGH RESEARCH CORES

Peptide Synthesis/Protein Sequencing
Biostatistics Core
Clinical Genetic Research Facility
Clinical Research Program
DNA Microarray Core (Seed)
DNA Sequencing Core
DNA Synthesis Core
Electrophysiology Core
Flow Cytometry Core
Glass Washing and Autoclaving Core
Knight Surgical Research Laboratory
Knockout Facility
Laser Capture Microscopy Core
Magnetic Resonance Imaging Core
Microscopy and Image Analysis Core
Microscopy Core (Brown)
Model Shop (Prototyping and Fabrication Facility)
Morphometry Analysis Center
Musculoskeletal Imaging Research Core
Neuroscience Microscope Core
Nucleic Acid Quantitation (Real-Time PCR) Core
Peptide Synthesis/Protein Sequencing Core
PET Core
Radiation Safety Core
Transgenic Core
Tumor Imaging Metrics Core
Vector Development and Production Core
Web Development Core
 

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Peptide Synthesis/Protein Sequencing Core
Director: Ashok Khatri
Location: Building 149 13th Street, Room 4314, Charlestown
 

Core Summary:

The Peptide Synthesis/Protein Sequencing Core provides peptide/protein services and
consultations for the synthesis of peptide and sequencing of peptides and proteins. The
Endocrine Unit and the Partners AIDS Research Center sponsor the Peptide Synthesis/
Protein Sequencing Core.

Personnel/Contact Information:

Director: Ashok Khatri
Phone: (617) 726-6187
Fax: (617) 726-7783
Email: akhatri@partners.org

Facilities and Equipment:

Location of Core:

The facility is located at Building 149 13th Street, Room 4314, Charlestown.

Major Equipment:

The Peptide Synthesis/Protein Sequencing Core located on the 4th floor of Charlestown
has been operational since 1995. The facility utilizes the following equipment:

For Peptide Synthesis:
Advanced Chemtech 396 Multiple Small Scale Peptide Synthesizer (3)
431A (Applied Biosystems) Large Scale Synthesizer (2)
Waters HPLCs for Analysis (2)
Waters Delta Prep HPLC System for large-scale purifications (1)
Waters Prep HPLC (1) for medium scale purifications
Beckman HPLC for Analysis (1)

Amino Acid Analysis:
Beckman Model 6300 (1)

Protein Sequencing:
P.E. Applied Biosystems Model 491A (1)
P.E. Applied Biosystems Microbore HPLC (2)

Mass Spectrometry:
Finnigan LCQ with a dedicated Michrom Bioresources microbore/capillary HPLC(1)

Services:

Peptide synthesis
Peptide purification
Conjugation of peptides to carrier proteins
Amino acid analysis
Amino acid sequencing
Mass spectroscopy