The Peptide Synthesis/Protein Sequencing Core provides
peptide/protein services and
consultations for the synthesis of peptide and sequencing of peptides and proteins.
The
Endocrine Unit and the Partners AIDS Research Center sponsor the Peptide Synthesis/
Protein Sequencing Core.
| Personnel/Contact
Information: |
Director: Ashok Khatri
Phone: (617) 726-6187
Fax: (617) 726-7783
Email: akhatri@partners.org
| Facilities
and Equipment: |
Location of Core:
The facility is located at Building 149 13th Street,
Room 4314, Charlestown.
Major Equipment:
The Peptide Synthesis/Protein Sequencing Core located
on the 4th floor of Charlestown
has been operational since 1995. The facility utilizes the following equipment:
For Peptide Synthesis:
Advanced Chemtech 396 Multiple Small Scale Peptide Synthesizer (3)
431A (Applied Biosystems) Large Scale Synthesizer (2)
Waters HPLCs for Analysis (2)
Waters Delta Prep HPLC System for large-scale purifications (1)
Waters Prep HPLC (1) for medium scale purifications
Beckman HPLC for Analysis (1)
Amino Acid Analysis:
Beckman Model 6300 (1)
Protein Sequencing:
P.E. Applied Biosystems Model 491A (1)
P.E. Applied Biosystems Microbore HPLC (2)
Mass Spectrometry:
Finnigan LCQ with a dedicated Michrom Bioresources microbore/capillary HPLC(1)
►Peptide
synthesis
►Peptide
purification
►Conjugation
of peptides to carrier proteins
►Amino
acid analysis
►Amino
acid sequencing
►Mass
spectroscopy
|