The Gene Modification Laboratory was established to integrate and launch new technologies
for rapid generation of gene-modified constructs and mutant mice for supporting mouse and
human genetics research programs in the Harvard / Partners community.

The laboratory currently applies the bacteria-based defective prophage recombineering
system for quickly engineering BAC DNA to generate BAC transgenic constructs with
markers, BAC knockout constructs, and plasmid knockout, conditional knockout and knock-in
constructs. The system relies mainly on PCR and homologous recombination in bacteria rather
than traditional restriction enzymes and DNA ligases. This offers not only very quick
generation of DNA constructs, but also abundant possibilities for desired DNA modifications
that may not be possible due to the limitation of restriction enzymes.

The laboratory also incorporates Embryonic Stem (ES) cell technologies including ES cell
culture and ES cell transfection and screening for generating gene-engineered mutant mice
for investigators.

In addition to carrying out its own independent research on generating mouse models for
human diseases, the laboratory also provides comprehensive services and consulting for
generating mouse models for human cancers or other diseases from DNA construct design
and generation, ES cell transfection and screening to final generation and screening of gene-
modified mice.

Scientific Director: Raju Kucherlapati, Ph.D.
Phone: (617) 525-4445
Fax: (617) 525-4440
Email: rkucherlapati@partners.org

Laboratory Director: David Conner, Ph.D.
Phone: (617) 432-6522
Fax: (617) 432-7832
Email: conner@receptor.med.harvard.edu

Location of Core:

The facility is located at 77 Avenue Louis Pasteur NRB, Rm 164 , Boston.

►DNA Constructs
►ES cell transfection
►ES cell screening
►Selection markers for free

To access the core, please contact David Conner at (617) 432-6522 or by email at
conner@receptor.med.harvard.edu. Please also see webiste at http://www.hpcgg.org.

http://www.hpcgg.org