DNA Sequencing Core
Director: Stephen Belmonte
Location: 38 Sidney Street, Cambridge
| Core Summary: |
The DNA Sequencing Core offers sequencing service in
a timely, economical, and reliable
manner. Results are made available to you over the internet as text-only files
and
chromatogram files which contain the fluorescent dye peaks the sequencer uses
to determine
base calls.
| Personnel/Contact Information: |
Director: Stephen Belmonte
Phone: (617) 726-0083
Fax: (617) 726-0077
Email: sbelmonte@ccib.mgh.harvard.edu
Other Staff:
Daniel Collette
Phone: (617) 726-0099
Fax: (617) 726-0077
Email: dcollette@ccib.mgh.harvard.edu
Karen Armstrong
Phone: (617) 726-5982
Fax: (617) 726-0077
Email: karmstrong@ccib.mgh.harvard.edu
| Facilities and Equipment: |
Location of Core:
The facility is located at 38 Sidney Street, Suite 100, Cambridge.
Major Equipment:
Two ABI 3730 XL DNA Analyzers
Qiagen BioRobot 3000
Two MJ Research DNA Engine Tetrad Thermal Cyclers
Six Hybaid MBS 0.2S Thermal Cyclers
ATR Multitron II Incubator Shaker
| Services: |
►Sequencing of DNA using Applied Biosystems Taq DyeDeoxy Terminator cycle sequencing
kits which utilize a fluorescently-labeled dideoxy-nucleotide chain termination method.
►Purification of plasmid DNA with Qiagen R.E.A.L. or Turbo Preps.
| Fee Schedule/Rates: |
| Service | MGH |
| Sequencing Reaction | $5 |
| High Throughput (more than 384/mo) | $4 |
| DNA Prep (per 96-well plate) | $192 |
| Getting Started: |
To register or download your results you should go to:
https://dnacore.mgh.harvard.edu/cgi-bin/login
Once you have registered and created a user account,
you will be able to generate a
bar-coded submission sheet to include with your sample.
dnacore@molbio.mgh.harvard.edu.
| Links to other outside Academic and Commercially Available Services: |
| Institution | Contact | Website |
| BWH | David Kwiatkowski (617) 355-9005 |
https://regepi.bwh.harvard.edu/SRS |
| Tufts University | Michael
Berne (617) 636-2422 |
http://tucf.org/dnasequencing-f.html |
| BIDMC | Ediane
Dutra (617) 667-0609 |
http://research.bidmc.harvard.edu/Corelabs/DNA Sequence.asp |
| Johns Hopkins University | Duane Bartley (410) 614-0196 |
http://www.daf.jhmi.edu/sequencing/index.htm |
| University of Pennsylvania | Tupan Ganguly (215) 573-723 |
http://www.med.upenn.edu/genetics/core-facs/dna-seq/ |
| Departmental Web Link: |
