DNA Sequencing Core
Director: Stephen Belmonte
Location: 38 Sidney Street, Cambridge
| Core Summary: |
The DNA Sequencing Core offers sequencing service in
a timely, economical, and reliable
manner. Results are made available to you over the internet as text-only files
and
chromatogram files which contain the fluorescent dye peaks the sequencer uses
to determine
base calls.
| Personnel/Contact Information: |
Director: Stephen Belmonte
Phone: (617) 726-0083
Fax: (617) 726-0077
Email: sbelmonte@ccib.mgh.harvard.edu
Other Staff:
Daniel Collette
Phone: (617) 726-0099
Fax: (617) 726-0077
Email: dcollette@ccib.mgh.harvard.edu
Karen Armstrong
Phone: (617) 726-5982
Fax: (617) 726-0077
Email: karmstrong@ccib.mgh.harvard.edu
Ulandt Kim
Phone: (617) 726-5982
Fax: (617) 726-0099
Email: ukim@ccib.mgh.harvard.edu
| Facilities and Equipment: |
Location of Core:
The facility is located at 38 Sidney Street, Suite 100, Cambridge.
Major Equipment:
Three ABI 3730 XL DNA Analyzers
Qiagen BioRobot 3000
One MJ Research DNA Engine Tetrad Thermal Cycler
Fourteen Hybaid MBS 0.2S Thermal Cyclers
ATR Multitron II Incubator Shaker
One Tecan Evo 200
| Services: |
- Sequencing of DNA using Applied Biosystems Taq DyeDeoxy Terminator cycle sequencing kits which utilize a fluorescently-labeled dideoxy-nucleotide chain termination method.
- Purification of plasmid DNA with Qiagen R.E.A.L. or Turbo Preps.
- Plasmid verification at 1- or 2-fold coverage for plasmids with a known sequence. The consensus sequence is provided in Genbank format with a variance table listing differences between the reference and consensus sequences.
- Primer walking for plasmid/templates up to 7kb
- PCR Purification
- Microsatellite analysis
| Fee Schedule/Rates: |
DNA Sequencing:
Partners Users |
External Users |
| $2.75 per sample | $4.80 per sample |
| $1.75 per sample for orders containing 96 samples in plate format* | $3.05 per sample for orders containing 96 samples in plate format* |
Microsatellite:
Partners Users |
External Users |
| $1.50 per sample | $2.62 per sample |
| $1.00 per sample for orders containing 96 samples in plate format* | $1.75 per sample for orders containing 96 samples in plate format* |
* Please note: These rates apply even if the samples fail to produce useful sequence data unless control samplese included with every run also fail. An order containing less than 96 samples will not be charged the lower rate even if submitted in a 96-well plate format.
| Getting Started: |
To register or download your results you should go to:
https://dnacore.mgh.harvard.edu/cgi-bin/login
Once you have registered and created a user account,
you will be able to generate a
bar-coded submission sheet to include with your sample.
dnacore@molbio.mgh.harvard.edu.
| Links to other outside Academic and Commercially Available Services: |
| Institution | Contact | Website |
| BWH | David Kwiatkowski (617) 355-9005 |
https://regepi.bwh.harvard.edu/SRS |
| Tufts University | Michael
Berne (617) 636-2422 |
http://tucf.org/dnasequencing-f.html |
| BIDMC | Ediane
Dutra (617) 667-0609 |
http://research.bidmc.harvard.edu/Corelabs/DNA Sequence.asp |
| Johns Hopkins University | Duane Bartley (410) 614-0196 |
http://www.daf.jhmi.edu/sequencing/index.htm |
| University of Pennsylvania | Tupan Ganguly (215) 573-723 |
http://www.med.upenn.edu/genetics/core-facs/dna-seq/ |
| Departmental Web Link: |
