Director: Mason Freeman
Location: 38 Sidney Street, Suite 100, Cambridge, MA 02139
| Core Summary: |
Summary: The CCIB DNA Core provides DNA sequencing, DNA synthesis and laboratory automation core services as well as special automation project design and implementation.
| Personnel/Contact Information: |
| Director: Mason Freeman, MD Phone: (617) 724-7704 Email: mfreeman@ccib.mgh.harvard.edu |
Sequencing Manager: Stephen Belmonte Phone: (617) 726-0083 Email: sbelmonte@ccib.mgh.harvard.edu |
Synthesis Manager: Andrew Moeller |
Automation Manager: John Darga |
Other Staff:
Sidney St. Facilities Manager: Douglas Rennie |
Customer Service: Karen Elmessadi |
Business/Billing: Daniel Collette |
Bioinformatics: Huajun Wang |
| Amy Avery Email: aavery@ccib.mgh.harvard.edu |
Adrian Idrizi Email: aidrizi@ccib.mgh.harvard.edu |
Ulandt Kim |
Melissa Wedemeyer |
| Facilities and Equipment: |
Location of Core:
The facility is located at 38 Sidney Street, Suite 100, Cambridge.
Major Equipment:
3730XL DNA Sequencers
MerMade DNA Synthesizer
Tecan Evo 150 Liquid Handling Workstation
Biomek FX Liquid Handling Core System
Perkin Elmer Janus Liquid Handling Workstation
Hudson RapidPick
Perkin Elmer TopCount NXT
| Services: |
- DNA Sequencing, Individual Tubes
- DNA Sequencing, Bulk
- Microsatellite Analysis, Individual Tubes
- Microsatellite Analysis, Bulk
- DNA Synthesis, 50nmol
- DNA Synthesis, 200nmol
- DNA Synthesis, 1umol
- Oligo Desalt (per oligo)
- 96-well Plasmid Preparations
- 96-well PCR Purifications
- Full PCR Processing
- cDNA Library Screens (coming soon)
- siRNA Library Screens (coming soon)
- Custom Projects
| FY12 User Fees (Effective 10/1/11 to 9/30/2012) : |
| Service | Internal Users |
External Users |
|
Partners |
Non-Partners AMC & Non-Profit |
For-Profit & Industry |
|
| Individual Sample Sequencing | $2.95 |
$4.25 |
$4.69 |
| Bulk Sequencing | $1.95 |
$2.81 |
$3.10 |
| Microsatellite | $1.50 |
$2.16 |
$2.39 |
| Bulk Microsatellite | $1.00 |
$1.44 |
$1.59 |
| 50 nanomole DNA Synthesis | $0.23 |
$0.33 |
$0.36 |
| 200 nanomole DNA Synthesis | $0.52 |
$0.75 |
$0.83 |
| 1 micromole DNA Synthesis | $0.98 |
$1.41 |
$1.56 |
| Oligo Desalting | $0.60 |
$0.86 |
$0.95 |
| 96-well Plasmid Preparations | $100.00 |
$144.00 |
$159.00 |
| 96-well PCR Purifications | $57.64 |
$83.00 |
$91.65 |
| Custom Automation Project | $99.00 |
$142.56 |
$157.41 |
| Microsatellite Analysis | $0.25 |
$0.36 |
$0.40 |
| PCR Clean-Up (small # samples) | $0.26 |
$0.37 |
$0.41 |
| High-GC Sequencing (add-on) | $0.47 |
$0.67 |
$0.74 |
| Mousetail Genotyping | $4.76 |
$6.86 |
$7.57 |
| Automation No Equipment | $45.63 |
$65.70 |
$72.55 |
| Custom Services | Internal Users |
External Users |
|
Partners |
Non-Partners AMC & Non-Profit |
For-Profit & Industry |
|
| Consumables | TBD |
+ 44% |
+59% |
| Labor Charge | $49.00/hour |
$70.56/hour |
$77.91/hour |
| Equipment Fees | $17.00/hour
|
$24.48/hour
|
$27.03/hour
|
| Getting Started: |
To access the core, please visit our website, https://dnacore.mgh.harvard.edu/cgi-bin/.
| Departmental Web Link: |
Center for Computational and Integrative Biology: http://ccib.mgh.harvard.edu
