CCIB DNA Core

Director: Nicole Stange-Thomann, Ph.D.
Location: 38 Sidney Street, Suite 100, Cambridge, MA 02139

 
 
Core Summary:

The Center for Computational and Integrative Biology (CCIB) DNA Core provides conventional Sanger DNA sequencing, Oligonucleotide Synthesis, custom molecular biology services, and high-throughput laboratory research automation services including project design and implementation.

Personnel/Contact Information:

Director:
Nicole Stange-Thomann, Ph.D.
Phone: (617) 726-0085
Email: nstange-thomann@ccib.mgh.harvard.edu

_____________________________________________

Faculty Directors:

Managers:

Faculty Director: Mason Freeman, M.D.
Phone: (617) 726-5906
Email: mfreeman@ccib.mgh.harvard.edu

Automation Manager: John Darga
Phone: (617) 726-0070
Email: jdarga@ccib.mgh.harvard.edu

Faculty Director (Automation): Brian Seed, Ph.D.
Phone: (617) 726-5975
Email: bseed@ccib.mgh.harvard.edu

Sequencing Manager: Stephen Belmonte
Phone: (617) 726-0083
Email: sbelmonte@ccib.mgh.harvard.edu

 

Synthesis Manager: Andrew Moeller
Phone: (617) 726-7901
Email: amoeller@ccib.mgh.harvard.edu

______________________________________________

Other Staff:

Business/Billing: Daniel Collette
Phone: (617) 726-0099
Email: dcollette@ccib.mgh.harvard.edu

Customer Service: Karen Elmessadi
Phone: (617) 726-5982
Email: dnacore@ccib.mgh.harvard.edu

Bioinformatics: Huajun Wang
Phone: (617) 643-0862
Email: hwang@ccib.mgh.harvard.edu

 

   

Adrian Idrizi
Email: aidrizi@ccib.mgh.harvard.edu

Amy Avery
Email: aavery@ccib.mgh.harvard.edu

Michael Mirisola
Email: mmirisola@ccib.mgh.harvard.edu
 

Facilities and Equipment:

Location of Core:
The facility is located at 38 Sidney Street, Suite 100, Cambridge.

Major Equipment:
ABI 3730xl DNA Analyzers
Eppendorf Mastercycler pro PCR Systems
Tecan Freedom EVO Automated Liquid Handler
Molecular Devices SpectraMax Plus
MerMadeV DNA/RNA Oligonucleotide Synthesizer
Hudson RapidPick
Perkin Elmer TopCount NXT Microplate Scintillation and Luminescence Counter
Beckman Coulter Biomek FX Laboratory Automation Workstation
Perkin Elmer Janus Automated Workstation
Beckman Coulter SAGIAN Core System
ABI 7300 Real-Time PCR System
ABI PRISM 7000 Sequence Detection System

Services:
  • Sanger DNA Sequencing (Individual Tubes, Bulk)
  • Microsatellite/MLPA Genotyping (Individual Tubes, Bulk)
  • Mouse Tail Genotyping
  • DNA Synthesis
  • 96-well Plasmid Preparations
  • 96-well PCR Purification
  • Custom Research Automation Services (including Real-Time PCR Assays, RNA and cDNA
    Preparation, cDNA Library Screening, siRNA Library Screening, Library Rearraying,
    Bacterial Colony Picking, Quantification and Normalization, Site-directed Mutagenesis, Protein
    Expression and Purification)
  • Custom Molecular Biology and Sequencing Services (including Plasmid Verification, Primer
    Walking, Midi/MaxiPlasmid Preparation, Isolation of bacterial genomic DNA, PCR, Long-Read
    Sanger Sequencing)
FY14 User Fees (Effective 10/01/13) :

Service
Internal Users
External Users
Partners
Non-Partners AMC & Non-Profit
For-Profit & Industry
DNA Sequencing, Individual Tubes
$3.18
$4.59
$5.06
DNA Sequencing, 96-well Format (Bulk)
$1.99
$2.87
$3.16
High-GC Sequencing (add-on)
$0.74
$1.07
$1.18
PCR Clean-Up, 96-well Format
$0.55
$0.79
$0.87
Microsatellite Genotyping, Individual Tubes
$1.62
$2.33
$2.57
Microsatellite Genotyping, 96-well Format (Bulk)
$1.05
$1.51
$1.67
Microsatellite Genotyping Analysis
$0.61
$0.88
$0.97
Custom Molecular Biology and Sequencing Services
Quote by Project
Quote by Project
Quote by Project
DNA Synthesis, 50 nmol
$0.24
$0.34
$0.38
DNA Synthesis, 200 nmol
$0.65
$0.93
$1.03
Oligo Desalting
$0.80
$1.15
$1.27
96-well Plasmid Preparations
$105.00
$151.20
$166.95
Mousetail Genotyping
$5.50
$7.92
$8.75
Addtl Genotyping PCR (add-on)
$2.50
$3.60
$3.98
Custom Research Automation Services
Quote by Project
Quote by Project
Quote by Project

*** Please contact the core for Quote by Project pricing ***

Getting Started:

To access the core, please visit our website, https://dnacore.mgh.harvard.edu/cgi-bin/

Departmental Web Link:

Center for Computational and Integrative Biology: http://ccib.mgh.harvard.edu

 

 

 
 
     
 

 

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