CCIB DNA Core

Director: Nicole Stange-Thomann, Ph.D.
Location: 38 Sidney Street, Suite 100, Cambridge, MA 02139

 
 
Core Summary:

The Center for Computational and Integrative Biology (CCIB) DNA Core provides conventional Sanger DNA sequencing, Oligonucleotide Synthesis, custom molecular biology services, and high-throughput laboratory research automation services including project design and implementation.

In addition, the core has developed a low-cost high-throughput approach for Complete Plasmid Sequencing which combines massively parallel sequencing with automated plasmid assembly. The early access phase of this new and unique service has started in July 2014; the official service launch is scheduled for September 2014.

Personnel/Contact Information:

Director:
Nicole Stange-Thomann, Ph.D.
Phone: (617) 726-0085
Email: nstange-thomann@ccib.mgh.harvard.edu

_____________________________________________

Faculty Directors:

Managers:

Faculty Director: Mason Freeman, M.D.
Phone: (617) 726-5906
Email: mfreeman@ccib.mgh.harvard.edu

Automation Manager: John Darga
Phone: (617) 726-0070
Email: jdarga@ccib.mgh.harvard.edu

Faculty Director (Automation): Brian Seed, Ph.D.
Phone: (617) 726-5975
Email: bseed@ccib.mgh.harvard.edu

Sequencing Manager: Stephen Belmonte
Phone: (617) 726-0083
Email: sbelmonte@ccib.mgh.harvard.edu

 

Synthesis Manager: Andrew Moeller
Phone: (617) 726-7901
Email: amoeller@ccib.mgh.harvard.edu

______________________________________________

Other Staff:

Business/Billing: Nishwetha Kumar
Phone: (617) 726-0069
Email: nkumar@ccib.mgh.harvard.edu

Business/Billing: Andrew Moeller
Phone: (617) 726-7901
Email: amoeller@ccib.mgh.harvard.edu

Bioinformatics: Huajun Wang
Phone: (617) 643-0862
Email: hwang@ccib.mgh.harvard.edu

Customer Service: Karen Elmessadi
Phone: (617) 726-5982
Email: dnacore@ccib.mgh.harvard.edu

   
Michael Mirisola
Email: mmirisola@ccib.mgh.harvard.edu
 

Facilities and Equipment:

Location of Core:
The facility is located at 38 Sidney Street, Suite 100, Cambridge.

Major Equipment:
ABI 3730xl DNA Analyzers
Eppendorf Mastercycler pro PCR Systems
Tecan Freedom EVO Automated Liquid Handler
Molecular Devices SpectraMax Plus
MerMadeV DNA/RNA Oligonucleotide Synthesizer
Hudson RapidPick
Perkin Elmer TopCount NXT Microplate Scintillation and Luminescence Counter
Beckman Coulter Biomek FX Laboratory Automation Workstation
Perkin Elmer Janus Automated Workstation
Beckman Coulter SAGIAN Core System
ABI 7300 Real-Time PCR System
ABI PRISM 7000 Sequence Detection System

Services:
  • Sanger DNA Sequencing (Individual Tubes, Bulk)
  • Microsatellite/MLPA Genotyping (Individual Tubes, Bulk)
  • 96-well PCR Purification (supporting Sanger Sequencing Service)
  • 96-well Plasmid Preparations
  • Complete Plasmid Sequencing with Assembly (Individual Tubes, Bulk)
  • DNA Synthesis
  • Mouse Tail Genotyping
  • Custom Research Automation Services (including cDNA Library Screening, siRNA Library Screening, Bacterial Colony Picking & Archiving, Library Rearraying, Quantification and Normalization, Site-directed Mutagenesis, Protein Expression and Purification)
  • Custom Molecular Biology and Sequencing Services (Click Here for more Details)
FY15 User Fees (Effective 10/01/14) :

Service
Internal Users
External Users
Partners
Non-Partners AMC & Non-Profit
For-Profit & Industry
DNA Sequencing, Individual Tubes
$3.26
$4.69
$5.18
DNA Sequencing, 96-well Format (Bulk)
$2.05
$2.95
$3.26
High-GC Sequencing (add-on)
$0.78
$1.12
$1.24
PCR Clean-Up, 96-well Format
$0.57
$0.82
$0.91
Microsatellite Genotyping, Individual Tubes
$1.67
$2.40
$2.66
Microsatellite Genotyping, 96-well Format (Bulk)
$1.08
$1.56
$1.72
Microsatellite Genotyping Analysis
$0.65
$0.94
$1.03
Custom Molecular Biology & Sequencing Services
Quote by Project
DNA Synthesis, 50 nmole scale (3-75 b)
$0.24
$0.35
$0.38
DNA Synthesis, 200 nmole scale (3-75 b)
$0.68
$0.98
$1.08
Oligo Desalting (3-75 b)
$0.80
$1.15
$1.27
96-well Plasmid Preparations
$108.00
$155.52
$171.72
Mousetail Genotyping
$5.50
$7.92
$8.75
Addtl Genotyping PCR (add-on)
$2.50
$3.60
$3.98
Custom Research Automation Services
Quote by Project
Complete Plasmid Sequencing (Individual)
$40.00
$57.60
$63.60
Complete Plasmid Sequencing, 96 Sample (Bulk)
$35.00
$50.40
$55.65
Outsourced DNA Synthesis & Purification Services (>75 b)

*** Please contact the core for Quote by Project pricing ***

Getting Started:

To access the core, please visit our website, https://dnacore.mgh.harvard.edu/new-cgi-bin/

Departmental Web Link:

Center for Computational and Integrative Biology: http://ccib.mgh.harvard.edu

 

 

 
 
     
 

 

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