 |
|
|
|
|
|
|
Co-Leaders: Immaculata De Vivo, David Kwiatkowski
Location: 77 Avenue Louis Pasteur, NRB 164, Boston
| Core Summary: |
The High-Throughput Polymorphism Detection Core provides
services to investigators who
are conducting molecular analyses of germline and somatic DNA collected as
part of cancer-
focused investigations. This Core provides high-throughput assays of specific
gene mutations
and single nucleotide polymorphisms (SNPs) in situations where previously defined
specific
nucleotide alterations are of interest.
| Personnel/Contact Information: |
Co-Leader: Immaculata De Vivo, Ph.D.
Phone: (617) 525-2142
Email: nhidv@channing.harvard.edu
Co-Leader: David Kwiatkowski, M.D., Ph.D.
Phone: (617) 525-2755
Email: dk@rics.bwh.harvard.edu
Other Staff:
Sequenom Genotyping Manager: Allison Brown, Ph.D.
Phone: (617) 768-8470
Email: abrown@rics.bwh.harvard.edu
Taqman Genotyping Manager: Hardeep Ranu, Ph.D.
Phone: (617) 432-4469
Email: hranu@hsph.harvard.edu
| Facilities: |
Location of Core:
The facility is located at 77 Avenue Louis Pasteur,
NRB 164, Boston and is a Unit of the
Harvard-Partners Center for Genetics & Genomics Genotyping Facility.
| Services: |
►SNP
Analysis using Taqman
►SNP
analysis using Sequenom
►DNA
Extraction, plating, and whole genome amplification
►Genotyping
using the SNPlex, Illumina, and Affymetrix technologies
| FY12 User Fees (Effective 10/1/11): |
Service |
Internal Users |
External Users |
|||
Partners DF/HCC Member |
Partners Non – DF/HCC Member |
AMC / Non-Profit DF/HCC Member |
Non-Partners AMC / Non-profit Non-DF/HCC Member |
For-profit & Industry |
|
SNP001: Design & Optimization (less than 700 samples) |
$224.29 |
$250.00 |
$224.29 |
$359.99 |
$397.49 |
SNP001.2: Design and Optimization (assays currently in stock) |
$358.88 |
$400.01 |
$358.88 |
$576.01 |
$636.01 |
SNP002: Design and Optimization (assays currently not in stock) |
$811.94 |
$905.00 |
$811.94 |
$1,303.19 |
$1,438.94 |
SNP003: Taqman Assay |
$0.57 |
$0.64 |
$0.57 |
$0.92 |
$1.01 |
SNP003.26: Taqman Assay |
$0.29 |
$0.33 |
$0.29 |
$0.47 |
$0.52 |
SNP004: OpenArray |
$0.14 |
$0.16 |
$0.14 |
$0.23 |
$0.25 |
EXT001: Sample Plating Only |
$0.92 |
$1.03 |
$0.92 |
$1.48 |
$1.64 |
EXT002: DNA Concentration (plate & quantitate DNA) |
$1.35 |
$1.51 |
$1.35 |
$2.17 |
$2.40 |
EXT003: Full DNA Extraction (extract, plate & quantitate DNA) |
$12.11 |
$13.50 |
$12.11 |
$19.44 |
$21.46 |
EXT005: Sample Standardization |
$2.74 |
$3.06 |
$2.74 |
$4.40 |
$4.86 |
EXT007: Qiagen Cleanup |
$4.79 |
$5.34 |
$4.79 |
$7.69 |
$8.49 |
WGA001: Whole Genome Amplification |
$12.34 |
$13.75 |
$12.34 |
$19.80 |
$21.87 |
Sequencing/GWAS Prep |
$4.76 |
$5.30 |
$4.76 |
$7.64 |
$8.43 |
Telomere |
$22.90 |
$25.52 |
$22.90 |
$36.75 |
$40.58 |
| Getting Started: |
To access the core for Sequenom services, please contact
Allison Brown at (617) 768-8470 or
by email at abrown@rics.bwh.harvard.edu.
To access the core for Taqman services, please contact
Hardeep Ranu at (617) 432-4469 or
by email at hranu@hsph.harvard.edu.
| Departmental Web Link: |
http://www.dfhcc.harvard.edu/core-facilities/high-throughput-polymorphism/
