DF/HCC RESEARCH CORES

DF/HCC High-Throughput Polymorphism Detection
Cancer Pharmacology Core
Cytogenetics Core
High-Throughput Polymorphism Detection Core
Recombinant Protein Expression and Purification Core
Specialized Histopathology Core
Tissue MicroArray Core
Tumor Imaging Metrics Core
  Search


 
High-Throughput Polymorphism Detection Core
Co-Leaders: Immaculata De Vivo, David Kwiatkowski
Location: 77 Avenue Louis Pasteur, NRB 164, Boston

 

Core Summary:

The High-Throughput Polymorphism Detection Core provides services to investigators who
are conducting molecular analyses of germline and somatic DNA collected as part of cancer-
focused investigations. This Core provides high-throughput assays of specific gene mutations
and single nucleotide polymorphisms (SNPs) in situations where previously defined specific
nucleotide alterations are of interest.

Personnel/Contact Information:

Co-Leader: Immaculata De Vivo, Ph.D.
Phone: (617) 525-2142
Email: nhidv@channing.harvard.edu

Co-Leader: David Kwiatkowski, M.D., Ph.D.
Phone: (617) 525-2755
Email: dk@rics.bwh.harvard.edu

Other Staff:

Sequenom Genotyping Manager: Allison Brown, Ph.D.
Phone: (617) 768-8470
Email: abrown@rics.bwh.harvard.edu

Taqman Genotyping Manager: Hardeep Ranu, Ph.D.
Phone: (617) 432-4469
Email: hranu@hsph.harvard.edu

Facilities:

Location of Core:

The facility is located at 77 Avenue Louis Pasteur, NRB 164, Boston and is a Unit of the
Harvard-Partners Center for Genetics & Genomics Genotyping Facility.

Services:

SNP Analysis using Taqman
SNP analysis using Sequenom
DNA Extraction, plating, and whole genome amplification
Genotyping using the SNPlex, Illumina, and Affymetrix technologies

FY11 User Fees (Effective 10/1/10-9/30/11):

 

Service
DF/HCC Members
Internal Users
External Users
Partners
Non Partners AMC & Non-profit
For-Profit & Industry
Design and Optimization (<700 samples)
$208
$250
$360
$397
Design and Optimization (assays currently in-stock)
$320
$400
$576
$636
Design and Optimization (assays currently not in-stock)
$753
$905
$1,303
$1,439
Taqman Assay – SNP003
$0.53
$0.63
$0.91
$1
Taqman Assay – SNP003.26
$0.26
$0.32
$0.46
$0.51
Biotrove
$0.14
$0.16
$0.24
$0.25
Sample Plating Only
$0.85
$1.02
$1.47
$1.62
DNA Concentration (Plate & Quantitate DNA)
$1.25
$1.51
$2.17
$2.40
Full DNA Extraction
$11.11
$13.35
$19.22
$21.23
WGA
$11.12
$13.36
$19.24
$21.24
WGA - Double
$15.02
$18.04
$25.97
$28.68
WGA – Plating and Storage
$0.87
$1.04
$1.50
$1.65
Qiagen Cleanup
$4.21
$5.05
$7.27
$8.03
GWAS Prep
$4.18
$5.02
$7.24
$7.98
Extractions X4
$23.35
$28.04
$40.38
$44.58
Telomere
$20.81
$25
$36
$39.75
Full DNA Extraction (double)
$14.97
$17.98
$25.89
$28.59
Sample Standardization
$2.53
$3.04
$4.38
$4.83

Getting Started:

To access the core for Sequenom services, please contact Allison Brown at (617) 768-8470 or
by email at abrown@rics.bwh.harvard.edu.

To access the core for Taqman services, please contact Hardeep Ranu at (617) 432-4469 or
by email at hranu@hsph.harvard.edu.

Departmental Web Link:

http://www.dfhcc.harvard.edu/core-facilities/high-throughput-polymorphism/