The High-Throughput Polymorphism Detection Core provides
services to investigators who
are conducting molecular analyses of germline and somatic DNA collected as
part of cancer-
focused investigations. This Core provides high-throughput assays of specific
gene mutations
and single nucleotide polymorphisms (SNPs) in situations where previously defined
specific
nucleotide alterations are of interest.
| Personnel/Contact
Information: |
Co-Leader: David Hunter, Sc.D., M.D.
Phone: (617) 525-2755
Email: nhdjh@channing.harvard.edu
Co-Leader: David Kwiatkowski, M.D., Ph.D.
Phone: (617) 525-2755
Email: dk@rics.bwh.harvard.edu
Other Staff:
Sequenom Genotyping Manager: Allison Brown, Ph.D.
Phone: (617) 768-8470
Email: abrown@rics.bwh.harvard.edu
Taqman Genotyping Manager: Hardeep Ranu, Ph.D.
Phone: (617) 432-4469
Email: hranu@hsph.harvard.edu
Location of Core:
The facility is located at 77 Avenue Louis Pasteur,
NRB 164, Boston and is a Unit of the
Harvard-Partners Center for Genetics & Genomics Genotyping Facility.
►SNP
Analysis using Taqman
►SNP
analysis using Sequenom
►DNA
Extraction, plating, and whole genome amplification
►Genotyping
using the SNPlex, Illumina, and Affymetrix technologies
1) SNP Analysis using Taqman
SNP start up fees:
◦ Fewer than 700 samples and assay not
in stock: $250.00
◦ 700-2000 samples and assay is in stock:
$400.00
◦ 700-2000 samples and assay not in stock:
$465.00
◦ 2000 samples or more and assay not in
stock: $905.00
Genotyping:
Taqman assay: $.60 per sample
Taqman assay (DF/HCC members): $.50 per sample
2) SNP analysis using Sequenom
SNP start up fees:
◦ $50.00 per SNP assay ordered, cost
covers assay design and oligo ordering, for anywhere
from 100-2000 DNA samples
Genotyping:
For orders of more than 20 SNP: $0.35 per SNP-genotype. For DF/HCC members,
$0.30
per SNP-genotype.
3) DNA Extraction, plating, and whole genome amplification
◦ Sample plating into
96 or 384 well plates: $.27 per sample
◦ DNA concentration (plate & quantitate
DNA) : $.74 per sample
◦ Full DNA extraction or whole genome
amplification: $10.00 per sample
All three of these are discounted at 10% for DF/HCC members.
4) Genotyping using the SNPlex, Illumina, and
Affymetrix technologies
To be determined. Will have 10% discount for DF/HCC members.
To access the core for Sequenom services, please contact
Allison Brown at (617) 768-8470 or
by email at abrown@rics.bwh.harvard.edu.
To access the core for Taqman services, please contact
Hardeep Ranu at (617) 432-4469 or
by email at hranu@hsph.harvard.edu.
http://www.dfhcc.harvard.edu/core-facilities/high-throughput-polymorphism/
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