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Co-Leaders: Immaculata De Vivo, David Kwiatkowski
Location: 77 Avenue Louis Pasteur, NRB 164, Boston
| Core Summary: |
The High-Throughput Polymorphism Detection Core provides
services to investigators who
are conducting molecular analyses of germline and somatic DNA collected as
part of cancer-
focused investigations. This Core provides high-throughput assays of specific
gene mutations
and single nucleotide polymorphisms (SNPs) in situations where previously defined
specific
nucleotide alterations are of interest.
| Personnel/Contact Information: |
Co-Leader: Immaculata De Vivo, Ph.D.
Phone: (617) 525-2142
Email: nhidv@channing.harvard.edu
Co-Leader: David Kwiatkowski, M.D., Ph.D.
Phone: (617) 525-2755
Email: dk@rics.bwh.harvard.edu
Sequenom Genotyping Manager: Allison Brown, Ph.D.
Phone: (617) 768-8470
Email: abrown@rics.bwh.harvard.edu
Taqman Genotyping Manager: Hardeep Ranu, Ph.D.
Phone: (617) 432-4469
Email: hranu@hsph.harvard.edu
| Facilities: |
Location of Core:
The facility is located at 77 Avenue Louis Pasteur,
NRB 164, Boston and is a Unit of the
Harvard-Partners Center for Genetics & Genomics Genotyping Facility.
| Services: |
►SNP
Analysis using Taqman
►SNP
analysis using Sequenom
►DNA
Extraction, plating, and whole genome amplification
►Genotyping
using the SNPlex, Illumina, and Affymetrix technologies
| FY13 User Fees (Effective 10/1/12): |
| Service | Internal Users | External Users | ||
| Partners Non-DF/HCC Member | Partners DF/HCC Member | Non-Partners AMC and Non-profit, Non-DF/HCC Member | For-profit and Industry, Non-DF/HCC Member (Includes 25% markup) | |
| Mouse ES Cell Quality Control | $250.00 | $195.00 | $360.00 | $496.88 |
| Quick Scan (human and mouse) | $320.00 | $249.60 | $460.80 | $636.00 |
| Partial Chromosome Analysis | $460.00 | $358.80 | $662.40 | $914.25 |
| Chromosome Analysis (human and mouse) | $670.00 | $522.60 | $964.80 | $1,331.63 |
| Unsuccessful Chromosome Study | $255.00 | $198.90 | $367.20 | $506.81 |
| Chromosome Harvest (no analysis) | $195.00 | $152.10 | $280.80 | $387.56 |
| Agilent aCGH (180K/244K/1M – human and mouse) | $930.00 | $725.40 | $1,339.20 | $1,848.38 |
| Affymetrix Cytoscan | $930.00 | $725.40 | $1,339.20 | $1,848.38 |
| Metaphase FISH | $800.00 | $624.00 | $1,152.00 | $1,590.00 |
| Interphase FISH | $745.00 | $581.10 | $1,072.80 | $1,480.69 |
| Interphase Tissue Microarray (TMA) | $1,520.00 | $1,185.60 | $2,188.80 | $3,021.00 |
| Additional slide for FISH hybridization | $295.00 | $230.10 | $424.80 | $586.31 |
| Additional probe | $25.00 | $19.50 | $36.00 | $49.69 |
| Unsuccessful FISH Study | $170.00 | $132.60 | $244.80 | $337.88 |
| Paraffin FISH | $790.00 | $616.20 | $1,137.60 | $1,570.13 |
| Multi-Plex FISH (M-FISH) | $1,185.00 | $924.30 | $1,706.40 | $2,355.19 |
| M-band FISH | $1,185.00 | $924.30 | $1,706.40 | $2,355.19 |
| Genomic DNA isolation (blood, cells, etc.) | $75.00 | $58.50 | $108.00 | $149.06 |
| BAC/Fosmid DNA preparation for FISH | $140.00 | $109.20 | $201.60 | $278.25 |
| DNA labeling | $140.00 | $109.20 | $201.60 | $278.25 |
| Purchase of BAC or fosmid (clones) | $160.00 | $124.80 | $230.40 | $318.00 |
| Culture initiation, maintenance and freezing | $305.00 | $237.90 | $439.20 | $606.19 |
| qPCR (per assay completed in quadruplicate) | $13.00 | $10.14 | $18.72 | $25.84 |
| Technical Cytogenetic Support and Consultation (per hour) | $70.00 | $54.60 | $100.80 | $139.13 |
| Getting Started: |
To access the core for Sequenom services, please contact
Allison Brown at (617) 768-8470 or
by email at abrown@rics.bwh.harvard.edu.
To access the core for Taqman services, please contact
Hardeep Ranu at (617) 432-4469 or
by email at hranu@hsph.harvard.edu.
| Departmental Web Link: |
http://www.dfhcc.harvard.edu/core-facilities/high-throughput-polymorphism/
