The High-Throughput Polymorphism Detection Core provides services to investigators who
are conducting molecular analyses of germline and somatic DNA collected as part of cancer-
focused investigations. This Core provides high-throughput assays of specific gene mutations
and single nucleotide polymorphisms (SNPs) in situations where previously defined specific
nucleotide alterations are of interest.

Co-Leader: David Hunter, Sc.D., M.D.
Phone: (617) 525-2755
Email: nhdjh@channing.harvard.edu

Co-Leader: David Kwiatkowski, M.D., Ph.D.
Phone: (617) 525-2755
Email: dk@rics.bwh.harvard.edu

Other Staff:

Sequenom Genotyping Manager: Allison Brown, Ph.D.
Phone: (617) 768-8470
Email: abrown@rics.bwh.harvard.edu

Taqman Genotyping Manager: Hardeep Ranu, Ph.D.
Phone: (617) 432-4469
Email: hranu@hsph.harvard.edu

Location of Core:

The facility is located at 77 Avenue Louis Pasteur, NRB 164, Boston and is a Unit of the
Harvard-Partners Center for Genetics & Genomics Genotyping Facility.

►SNP Analysis using Taqman
►SNP analysis using Sequenom
►DNA Extraction, plating, and whole genome amplification
►Genotyping using the SNPlex, Illumina, and Affymetrix technologies

1) SNP Analysis using Taqman

SNP start up fees:
     ◦   Fewer than 700 samples and assay not in stock: $250.00
     ◦   700-2000 samples and assay is in stock: $400.00
     ◦   700-2000 samples and assay not in stock: $465.00
     ◦  2000 samples or more and assay not in stock: $905.00

Genotyping:
   Taqman assay: $.60 per sample
   Taqman assay (DF/HCC members): $.50 per sample

2) SNP analysis using Sequenom

SNP start up fees:
     ◦   $50.00 per SNP assay ordered, cost covers assay design and oligo ordering, for anywhere
from 100-2000 DNA samples

Genotyping:
   For orders of more than 20 SNP: $0.35 per SNP-genotype.  For  DF/HCC members, $0.30
per SNP-genotype.

3) DNA Extraction, plating, and whole genome amplification
    ◦    Sample plating into 96 or 384 well plates: $.27 per sample
    ◦    DNA concentration (plate & quantitate DNA) : $.74 per sample
    ◦    Full DNA extraction or whole genome amplification: $10.00 per sample

 All three of these are discounted at 10% for DF/HCC members.

4) Genotyping using the SNPlex, Illumina, and Affymetrix technologies
To be determined.  Will have 10% discount for DF/HCC members.

To access the core for Sequenom services, please contact Allison Brown at (617) 768-8470 or
by email at abrown@rics.bwh.harvard.edu.

To access the core for Taqman services, please contact Hardeep Ranu at (617) 432-4469 or
by email at hranu@hsph.harvard.edu.

http://www.dfhcc.harvard.edu/core-facilities/high-throughput-polymorphism/