The High-Throughput Polymorphism Detection Core provides
services to investigators who
are conducting molecular analyses of germline and somatic DNA collected as
part of cancer-
focused investigations. This Core provides high-throughput assays of specific
gene mutations
and single nucleotide polymorphisms (SNPs) in situations where previously defined
specific
nucleotide alterations are of interest.
| Personnel/Contact
Information: |
Co-Leader: Immaculata De Vivo, Ph.D.
Phone: (617) 525-2142
Email: nhidv@channing.harvard.edu
Co-Leader: David Kwiatkowski, M.D., Ph.D.
Phone: (617) 525-2755
Email: dk@rics.bwh.harvard.edu
Sequenom Genotyping Manager: Allison Brown, Ph.D.
Phone: (617) 768-8470
Email: abrown@rics.bwh.harvard.edu
Taqman Genotyping Manager: Hardeep Ranu, Ph.D.
Phone: (617) 432-4469
Email: hranu@hsph.harvard.edu
Location of Core:
The facility is located at 77 Avenue Louis Pasteur,
NRB 164, Boston and is a Unit of the
Harvard-Partners Center for Genetics & Genomics Genotyping Facility.
►SNP
Analysis using Taqman
►SNP
analysis using Sequenom
►DNA
Extraction, plating, and whole genome amplification
►Genotyping
using the SNPlex, Illumina, and Affymetrix technologies
| FY13 User Fees (Effective 10/1/12): |
| Service |
Internal Users |
External Users |
| Partners Non-DF/HCC Member |
Partners DF/HCC Member |
Non-Partners AMC and Non-profit, Non-DF/HCC Member |
For-profit and Industry, Non-DF/HCC Member (Includes 25% markup) |
| Mouse ES Cell Quality Control |
$250.00 |
$195.00 |
$360.00 |
$496.88 |
| Quick Scan (human and mouse) |
$320.00 |
$249.60 |
$460.80 |
$636.00 |
| Partial Chromosome Analysis |
$460.00 |
$358.80 |
$662.40 |
$914.25 |
| Chromosome Analysis (human and mouse) |
$670.00 |
$522.60 |
$964.80 |
$1,331.63 |
| Unsuccessful Chromosome Study |
$255.00 |
$198.90 |
$367.20 |
$506.81 |
| Chromosome Harvest (no analysis) |
$195.00 |
$152.10 |
$280.80 |
$387.56 |
| Agilent aCGH (180K/244K/1M – human and mouse) |
$930.00 |
$725.40 |
$1,339.20 |
$1,848.38 |
| Affymetrix Cytoscan |
$930.00 |
$725.40 |
$1,339.20 |
$1,848.38 |
| Metaphase FISH |
$800.00 |
$624.00 |
$1,152.00 |
$1,590.00 |
| Interphase FISH |
$745.00 |
$581.10 |
$1,072.80 |
$1,480.69 |
| Interphase Tissue Microarray (TMA) |
$1,520.00 |
$1,185.60 |
$2,188.80 |
$3,021.00 |
| Additional slide for FISH hybridization |
$295.00 |
$230.10 |
$424.80 |
$586.31 |
| Additional probe |
$25.00 |
$19.50 |
$36.00 |
$49.69 |
| Unsuccessful FISH Study |
$170.00 |
$132.60 |
$244.80 |
$337.88 |
| Paraffin FISH |
$790.00 |
$616.20 |
$1,137.60 |
$1,570.13 |
| Multi-Plex FISH (M-FISH) |
$1,185.00 |
$924.30 |
$1,706.40 |
$2,355.19 |
| M-band FISH |
$1,185.00 |
$924.30 |
$1,706.40 |
$2,355.19 |
| Genomic DNA isolation (blood, cells, etc.) |
$75.00 |
$58.50 |
$108.00 |
$149.06 |
| BAC/Fosmid DNA preparation for FISH |
$140.00 |
$109.20 |
$201.60 |
$278.25 |
| DNA labeling |
$140.00 |
$109.20 |
$201.60 |
$278.25 |
| Purchase of BAC or fosmid (clones) |
$160.00 |
$124.80 |
$230.40 |
$318.00 |
| Culture initiation, maintenance and freezing |
$305.00 |
$237.90 |
$439.20 |
$606.19 |
| qPCR (per assay completed in quadruplicate) |
$13.00 |
$10.14 |
$18.72 |
$25.84 |
| Technical Cytogenetic Support and Consultation (per hour) |
$70.00 |
$54.60 |
$100.80 |
$139.13 |
To access the core for Sequenom services, please contact
Allison Brown at (617) 768-8470 or
by email at abrown@rics.bwh.harvard.edu.
To access the core for Taqman services, please contact
Hardeep Ranu at (617) 432-4469 or
by email at hranu@hsph.harvard.edu.
http://www.dfhcc.harvard.edu/core-facilities/high-throughput-polymorphism/
|