Co-Directors: James Decaprio, Laura Corral
Location: 21/27 Burlington Avenue, 4th Floor, Boston
| Core Summary: |
The Monoclonal Antibody Core produces monoclonal antibodies
directed against antigens of
interest to DF/HCC investigators. These monoclonal antibodies are used for
basic research,
drug discovery, and clinical applications including diagnosis, surrogate markers
for disease
status, response to therapy, and drug toxicity.
| Personnel/Contact Information: |
Co-Director: James Decaprio, M.D.
Phone: (617) 632-3825
Fax: (617) 632-4760
Email: james_decaprio@dfci.harvard.edu
Co-Director: Laura Corral, Ph.D.
Phone: (617) 632-2209
Email: laura_corral@dfci.harvard.edu
| Facilities: |
Location of Core:
The facility is located at DFCI, 21/27 Burlington Avenue, 4th Floor, Boston.
| Services and Fee Schedule/Rates: |
| Services | DF/HCC Members |
|
| Immunizations | ||
| 3 mice (includes 3 i.p. inj/mouse & collection of blood sera) | $1,200 |
|
| 3 rats (includes 3 i.p. inj/rats & collection of blood sera) | $1,800 |
|
| Additional 2 injections with collection of blood sera | $250 |
|
| Fusions | ||
| Mouse | $1,500 |
|
| Rat | $1,700 |
|
| Screens for Sera Testing, Fusions and Clones | ||
| Western Blot (per gel run) | $120 |
|
| Direct ELISA (per plate set up) | $120 |
|
| Sandwich ELISA (per plate set up) | $180 |
|
| IHC (immunohistochemistry) (per plate or set of 8 slides) | $250 |
|
| IF (immunofluorescence) (per plate or set of 8 slides) | $350 |
|
| Flow Cytometry (per plate) | $350 |
|
| Subcloning and Related Services | ||
| Subcloning per parental hybridoma | $250 |
|
| Cryopreservation (2 vials/hybridoma) | $55 |
|
Thawing & expansion of hybridomas or cell lines |
$55 |
|
| Isotyping of antibody | $30 |
|
| Adaptation of clone to serum-free media | $80 |
|
| Hybridoma Productivity Assay | $75 |
|
| Mycoplasma testing of hybridomas or other cell lines | $85 |
|
| Antibody Production | ||
| 1-2 mL | $10 |
|
| 5-10 mL | $25 |
|
| 15-20 mL | $50 |
|
| Between 20 and 50 mL | $100 |
|
| Between 50 and 200 mL | $400 |
|
| Between 200 and 500 mL | $600 |
|
| Between 0.5 and 1 L | $800 |
|
| Integra flask | $1,200
|
|
| Antibody Purification | Protein A |
Protein
G |
| Up to 500 mL | $525 |
$625 |
| Between 0.5 and 1 L | $725 |
$825 |
| Between 1 and 5 L | $1,300 |
$1,400 |
| Between 5 and 10 L | $2,500 |
$2,600 |
| Molecular Biology and Protein Expression | ||
| Cloning of one fragment into one expression vector | $750 |
|
| Cloning of one fragment into two expression vectors | $1,250 |
|
| Expression of one construct in a bacterial expression vector | $975 |
|
| Expression of one construct in a mammalian expression vector (3 plates) | $1,250 |
|
| Expression of one construct in a mammalian expression vector (10 plates) | $2,500 |
|
| Expression of one construct in a mammalian expression vector (15 plates) | $3,750 |
|
| Additional Costs/Services | ||
| Splenectomy & cryopreservation of spleens (2 vials/spleen) | $250 |
|
| Collection of final bleed/animal | $50 |
|
| Getting Started: |
To access the core, please contact Laura Corral at (617)
632-2209 or by email at
laura_corral@dfci.harvard.edu.
| Departmental Web Link: |
http://www.dfhcc.harvard.edu/core-facilities/monoclonal-antibody/
