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Office Hours:
8:00 a.m. - 5:30 p.m., Monday through Friday
8:00 a.m. - 2:00 p.m., Saturday
Telephone:
617-732-7510 General Pathology Office
617-732-7536 Women's & Perinatal Pathology
617-732-7514 Chairman's Office
617-732-6791 Administration
617-732-7532 Neuropathology
617-732-4715 Cytology
617-732-7981 Cytogenetics
Nights, Weekends, and Holidays
For the on-call Resident or Fellow please page the following:
Chief Resident BB - 11661
Senior Resident on Surgical Service (daytime only) BB - 11514
Daytime Frozen Sections BB - 11601
Daytime Gyn-Path Frozens BB - 11805
Nighttime Frozen Sections BB 11554
Weekend Frozen Sections BB 32765
Neuropath BB - 11660
Hematopath (daytime only) BB - 11659
Saturday on-call BB - 11285
Sunday on-call BB 11765
Requisition Forms: Available at the main
office on Amory 3, Rm. AL3-117; ample supply also available in operating
rooms, endoscopy suites, cardiac catherization labs, dermatology,
etc.
Specimen Delivery: Laboratories on Amory 3: Surgical Pathology
(Room # 106), Women's & Perinatal (Room # 129)
Emergency Procedures: Page Resident-On-Call
through hospital page operator, 617 732-6660.
Special Procedures: Infectious (HIV, Jacob-Creutzfeld,
hepatitis, tuberculosis) and hazardous (radioisotopic-contaminated)
specimens must be so labeled and brought to the attention of the pathologist.
Subspecialty Services Available in Addition
to General Surgical & Obstetric/Gynecologic Pathology:
- Breast pathology
- Cardiovascular pathology
- Dermatopathology
- Gastrointestinal pathology
- Hematopathology
- Male genitourinary pathology
- Molecular diagnostics
- Neuropathology
- Perinatal pathology
- Pulmonary pathology
- Renal pathology
- Soft tissue tumor pathology
Consultations on Outside Specimens
Review of material by one or more of our pathologists with comprehensive
adjunct special studies may be requested at any time on slides and/or
blocks of histo- or cytopathologic material previously prepared and/or
diagnosed at an outside institution.
Special Studies Available
- Histology special stains
- Electron microscopy (transmission)
- Immunohistochemistry (immunofluorescence and immunoperoxidase
techniques)
- In-situ hybridization
- Reverse transcriptase - PCR
- Cytogenetics
Availability of Results
Results of diagnostic pathology interpretations are available to the
clinician generally within 48 hours for small biopsies and excisional
specimens and within 72 hours for large resections. Rush services
are available upon special request. Pathology reports are availble
to clinicians on the BWH Hospital Information System (Clinical Information)
via the patient's name and hospital identification number. Preliminary
reports are often available in BICS before the final report.
Frozen Section-Intraoperative Consultations
Frozen sections 1) provide rapid gross or microscopic diagnoses
to guide intra- or perioperative patient management, and 2) allow
rapid processing tissue for special studies to be used for diagnosis
(e.g., cytogenetics or EM) or treatment (hormone receptor analysis).
The frozen section room is staffed by a senior pathologist and a resident
at all times.
Specimen Submission
Submit routine biopsy tissue specimen in biopsy container with
buffered formalin, in volume at least ten times the volume of the
specimen in a tightly capped leak proof container. Some biopsy specimens
either require fixation other than fomalin, e.g., bone marrow biopsies
are fixed in Zenker's acetic fixative (indicate time placed in fixative
on container), or the entire specimen (large specimens, hematologic
specimens as lymph nodes biopsied for lymphoma) or parts of specimens
(renal biopsies, soft tissue tumors) must be submitted fresh (refrigerate,
transport outside cases promptly on "wet" ice to pathology);
additional detail is provided below. Print patient's name/identification
on specimen container. Submit with a completed Tissue Pathology Requisition,
including clinical history.
The specimen should not be burned by cautery or crushed by forceps,
hemostats, or other instruments. The specimen should not be washed
with water; nor should it be allowed to dry out. When individual identity
of multiple biopsies is required, place each specimen into a separate,
properly labeled bottle.
The type of fixative must be identified on the container. The time
of placing the specimen in the fixative should be included when appropriate
(e.g., for fixatives containing mercury, such as Zenker's, or if rush
processing is requested).
If orientation will be important for the evaluation of the specimen
(e.g., malignant tumors for which re-excision of positive or close
margins is possible), preferably the surgeon should orient the specimen
with a pathology resident in the frozen section room or else external
markers must be used to provide this information for the pathologist.
Specimens for microbiologic culture are best taken in the operating
room, and cannot be done on a fixed specimen.
Specimens Requiring Special Techniques
| Specimens for electron microscopy |
Rapidly fixed in glutaraldehyde; specimen must be less than
0.1 cm thick |
Preservation of subcellular detail |
Breast carcinomas |
Fresh (refrigerate) |
Inking of specimens and possible hormone receptor and flow cytometric
analysis on fresh tissue. |
Bone marrow |
Zenker's acetic fixative |
Optimal cytologic detail and decalcification |
Lymphomas |
Fresh (refrigerate) |
Flow cytometric analysis, DNA analysis, cytogenetics, or frozen
for marker studies |
Skin biopsies: Bullous disease or SLE |
Fresh (refrigerate) |
Immunofluorescence |
Kidney biopsies |
Fresh (refrigerate) |
Immunofluorescence and EM |
Gout |
Fix in 100% ethanol for anaqueous processing |
Maintain crystals |
Liver: Acute fatty liver |
Fresh (refrigerate) |
Frozen section for microvesicular fat |
Liver: Copper |
Special |
Specimen must be handled without metal to avoid trace contamination
with metals |
Sarcomas, mesotheliomas, small round blue cell tumors, unusual
tumors |
Fresh, sterile (refrigerate) |
Cytogenetics, frozen tissue, special fixatives, e.g. for electron
microscopy |
Bullets or other "medicolegal" specimens |
Direct transfer |
Must maintain chain of custody within the hospital. |
Suspected infectious process |
Fresh, sterile |
Microbiologic culture |
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